Using macrophage cell culture to study the processes that result in removal of blood from bruises

Macrophages are responsible for removing the extravasated blood that forms a bruise. Aspects of this process, such as how long it takes erythrocytes to be phagocytosed and iron demonstrated using Perls stain, not established. This work sought assess possibility macrophages in cell culture address some unknowns processes resolution Human peripheral monocytes were obtained from buffy coats provided by Australian Red Cross Blood Service. Monocytes isolated adherence tissue plastic differentiated into macrophages. clot was added with Dulbecco’s Modified Eagle Medium (DMEM) combination Fetal Calf Serum (FCS) and/or Heat inactivated human serum (HI HUS). After 24 hours supernatant washed off replaced fresh medium. It noted red cells could seen 4 days, showed prominent blue colour stain indicating presence hemosiderin. pilot study has shown system, can breakdown produce model system may used investigate behind bruises.